Bispecific antibodies Research Paper Example | Topics and Well Written Essays - 5750 words

Bispecific antibodies - Research Paper Example In recent years, antibody therapy has become a new treatment modality for tumor patients, although the majority of responses are only partial and not long lasting. Based on evidence that effecter- cell-mediated mechanisms significantly contribute to antibody efficacy in vivo, several approaches are currently perused to improve the interaction between Fc receptor-expressing effecter cells and tumor target antigens. With this purpose the invention of monoclonal antibodies in vivo started. In the initial phase the results were not satisfactory and these antibodies in trial in vivo showed a only 20% clearance of the tumor cells but after letting it go through different formatting processes it went up to achieving 80% clearance. Bispecific antibodies have got 2 hinging sites which are specific for getting attached to immune recruiting cells and also to target antigens which are mostly transformed cells. "Bispecific antibodies (BsAb) can, by virtue of combining two binding specificities, i mprove the selectivity and efficacy of antibody-based treatment of human disease. Recent studies underline the importance of both the 'anti-trigger' and 'anti-target' modalities of BsAb for therapeutic efficacy". (Spriel, A.B., Ojik, H.H.V, & Winkel, J.G.J. 2000). There has always been an issue of side effects when it comes to cancer therapy and a lot of patients would not even go for therapies due the bad side effects. In the past few decades things have started improving an the standard mode of ontological therapies which were chemotherapy and radiation it is now switching more towards treatment of cancer with more of antibiotic and immunoglobulin. This has brought a lot of hope for future success in getting a strong hold of cancer with fewer emergences of side effects. Bispecific antibodies do not occur in nature and they need to be synthesized in vivo, through either recombinant DNA, or cell fusion technique. "Bispecific antibodies have been manufactured by fusing the DNA encodi ng a single chain antibody (ScFv) after the C terminus (CH3-ScFv) or after the hinge (Hinge-ScFv) with an antibody of a different specificity. The fusion protein is expressed by gene transfection in the context of a murine variable region. Transfectomas secrete a homogeneous population of the recombinant antibody with two different specificities, one at the N terminus (anti-dextran) and one at the C terminus (anti-dansyl). The CH3-ScFv antibody, which maintains the constant region of human lgG3, has some of the associated effectors functions such as long half-life and Fc receptor binding. The Hinge-ScFv antibody which lacks the CH2 and CH3 domains has no known effectors functions". (Coloma, M.J. &'Morrison, S.L. 1997). Production of Bispecific antibodies has been a challenging task but has still been encouraged because of the advantages it has towards treating cancer with less side effects. There are still some disadvantaged of cost and failure rate, and future challenges and tasks are also to be taken in consideration. We will have an overview of the rational of producing Bispecific antibo

Practice in creating, justifying, and explaining a decision-making Essay

Practice in creating, justifying, and explaining a decision-making proposal. All elements involved in the creation of this proposal, from problem definition through action plan - Essay Example It is believed that external head shall also provide required motivation and energy to the staff members. The company is a small branch of a banking concern located in a sound locality with a good track record. The branch was fully functional and operated under central supervision located within the city limits. The problem associated with this branch was high level of employee attrition. It was observed that employees left within 6 months of joining at different posts. The problem was critical for the post of operations manager of the bank. The bank branch had department strength of 6 operation managers, where the highest official had at most 12 months of experience with the branch. The event was triggered by retirement of the key operations head of the bank who had been in the position for over 25 years; and by a simultaneous change of department structure within the branch, which required a larger operations team. It was found that the employees were regular at their work for initial few weeks and their work quality was high during this time. After about four weeks on an average, the employee absenteeism started to reflect and their work quality also suffered. The relationship between different members of the department and ultimate head of the department was generally cordial. It was also seen that operations staff shared happy times with other staff of the bank as well as the branch manager. The underlying rationale behind this attrition is improper induction training provided to the incumbent and the lack of proper guidance at work. This resulted in poor work quality and less rewards (Tillman & Cassone, 2012). This had also entailed lack of job satisfaction, leading to attrition from work. The problem is more acute because most of the staff has been trained by less experienced members and the department majorly lacks in terms of knowledge pertaining to functioning of the banking process. The objective of this paper is